Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and COL4A2 gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.

Article Snippet: LC3 Polyclonal antibody (14600-1-AP), p62 Polyclonal antibody (80294-1-RR), PINK1 Polyclonal antibody (23274-1-AP), Parkin Polyclonal antibody (14060-1-AP), E-cadherin Polyclonal antibody (20874-1-AP), N-cadherin Polyclonal antibody (No.: 22018-1-AP), Vimentin Polyclonal antibody (10366-1-AP), COL4A2 Polyclonal antibody (55131-1-AP), FN1 Monoclonal antibody (66042-1-Ig), β-Actin Polyclonal antibody (20536-1-AP), and α-Tubulin Polyclonal antibody (11224-AP) were from Proteintech group Co., Ltd (Wuhan, China).

Techniques: Staining, Expressing, Immunohistochemistry, Western Blot, Control, Functional Assay, RNA Sequencing

Journal: Journal of Pharmaceutical Analysis

Article Title: Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice

doi: 10.1016/j.jpha.2025.101273

Figure Lengend Snippet: Identification and validation of potential signature molecules affected by liraglutide (Lira). (A) Protein-protein interaction (PPI) analysis of differential proteins and glycoproteins in major pathways. Node size reflects degree value, with red representing glycoproteins and blue representing proteins. Color-coded segments show pathway associations. (B) Messenger RNA (mRNA) expression of Acaa2 , Lamc1 , and Col4a2 (for proteins), and Acox1 , Gclc , and Shmt2 (for glycoproteins). (C) Protein expression of laminin subunit gamma-1 (LAMC1), collagen type IV alpha-2 chain (COL4A2), and acetyl-CoA acyltransferase 2 (ACAA2) (for proteins), and glutamate-cysteine ligase catalytic subunit (GCLC) (for glycoproteins). Data is presented as mean ± standard deviation (SD) ( n = 3 per group). P values were calculated using Student's t -test or one-way analysis of variance (ANOVA) with Tukey's comparisons. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. ns: not significant. PPAR: peroxisome proliferators-activated receptor; ECM: extracellular matrix; ND: normal diet; HFD: high-fat diet; HFDL: HFD + Lira; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The primary antibodies used in our study were as follows: laminin subunit gamma-1 (LAMC1) (1:8000, 67706-1-Ig; Proteintech Group, Inc., Wuhan, China), collagen type IV alpha-2 chain (COL4A2) (1:5000, 55131-1-AP; Proteintech Group, Inc.), acetyl-CoA acyltransferase 2 (ACAA2) (1:5000, 11111-1-AP; Proteintech Group, Inc.), glutamate-cysteine ligase catalytic subunit (GCLC) (1:6000, 12601-1-AP; Proteintech Group, Inc.), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:100000, 60004-1-Ig; Proteintech Group, Inc.), and α-tubulin (1:6000, PTM-5001; PTM BIO, Hangzhou, China).

Techniques: Biomarker Discovery, Expressing, Standard Deviation